Head: Pavel Souček, Ph.D.
email: psoucek@szucz, phone: +420-267082711
Basic information about Toxicogenomics Unit
Unit is involved in the following studies:
(1) Role of biotransformation enzymes in chemical carcinogenesis and genotoxicity.
The major aim of this program is to define the role of individual susceptibility in toxicity of chemicals and foresee applicability of assessment of genetic polymorphisms in biotransformation enzymes as risk factors in genotoxicity and subsequent onset of cancer. Cohorts of occupationally exposed workers and patients with breast, colorectal, and pancreatic carcinomas are currently under study.
(2) Expression of biotransformation genes and enzymes as promising markers of prognosis and prediction of therapy outcome in cancer patients.
Preparations of tumors and adjacent tissue without morphological signs of tumor cells from cancer patients are analyzed for expression of biotransformation enzymes (e.g. cytochromes P450s and ABC transporters) in cancer patients.
(3) Mechanisms of action of anticancer drugs and ways to enhance their efficacy.
This program is devoted to the study of taxane and antracycline drugs frequently used for treatment of breast cancer. Enzymes inactivating these drugs by direct metabolism and ABC transporters which actively transport them outside of cancer cells are studied. New promising derivatives of taxanes are under characterization at present. Models including sensitive and resistant stable cell lines derived from human breast tumors are employed.
Staff activities constitute of:
- Lecturing at Faculty of Natural History, Charles University in Prague
- Supervision of graduate students (Lucie Pospíšilová defended diploma thesis at Charles University in 1998; Jana Šarmanová, 1999; Tereza Borovanová, 2000; Šárka Matějková-Ozgová, 2000; Radka Václavíková, 2001; Jiří Hodis, 2005; Lenka Grosová, 2006; Lenka Maršáková, 2008; Karolína Letáková-Perglerová, 2010; Martina Protivová, 2011, Tereza Kunická, 2011)
- Supervision of doctoral students (Jana Šarmanová-Stříbrná, defended doctoral thesis at Charles University in 2004; Radka Václavíková, 2006; Eliška Kondrová, 2008; David Vrána, 2011; Beatrice Mohelníková-Duchoňová, 2012), Ing. Ivona Hlavatá, Ing. Miluše Hubáčková, Veronika Brynychová, MSc; Viktor Hlaváč, MSc and Tereza Kunická, MSc, conduct their studies at GB at present
- Reviewing of grant applications for Internal Grant Agency of Ministry of Health of CR, Grant Agency of CR, Grant Agency of Charles University, Grant Agency of Czech Academy of Sciences
- Reviewing manuscripts for Cancer Letters, International Journal of Cancer, Chemical Research in Toxicology, Cancer Genetics and Cytogenetics, Human Molecular Genetics, Leukemia and Lymphoma, Neuroendocrinology Letters, Pharmacogenetics and Genomics, European Journal of Pharmacology, Haematologica and Chemical Papers., and others
- Reviewing proposals for usage of genetically modified organisms from the view of labor safety
- Memberships in ISSX, AACR, UICC, Czech Society of Biochemistry and Molecular Biology, Czech Medical Society, Czech Society of Medical Genetics, Czech Society of Xenobiochemistry, Czech Society of Clinical and Experimental Pharmacology and Toxicology
- Membership in Habilitation Board of Faculty of Science, Charles University, Prague, CR; and membership in Commission for doctoral exams and defense of doctoral theses at Faculty of Science, Charles University